University of Texas at Dallas Sequences Using Mass Spectrometry Discussion
Question Description
I’m working on a biochemistry multi-part question and need an explanation and answer to help me learn.
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The first peptide is IEGKADST. Please draw the structure (pH=7) and indicate thedifferent possible y and b daughter ions that may result from running tandem MS-MS onthe structure. What is the sequence of the y3 and b6 ions?b. The second peptide MSYGRLISKAETLARAGW is cleaved by the reaction with trypsin.Then the products of the digestion are subjected to ESI-TOF MS in positive ion mode withthe peptides in a pH 2 solution. What peptide fragments will result and what are their m/z?c. For the products of the digestion in question 2b, which product will reach the detectorfirst? Explain why? The peptide KTDLRFQSSAVMALQEACEAY is digested with thermolysin. Then theproducts of the digestion are subjected to ESI-TOF MS in positive ion mode with thepeptides in a pH 2 solution. What peptide fragments will result and what are their m/z?
